Journal: Science Advances

Article Title: Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

doi: 10.1126/sciadv.abo7868

Figure Lengend Snippet: ( A ) Safranin O/fast green (SO/FG) staining of knee articular cartilage at 0, 2, 4, and 8 weeks after operation. Scale bar, 100 μm. ( B ) OARSI grade of knee articular cartilage. ( C ) Three-dimensional images of the sagittal plane of medial tibial subchondral bone at 0, 2, 4, and 8 weeks after ACLT. Scale bar, 500 μm. ( D ) Micro-CT quantitative analysis of tibial subchondral bone, BV/TV (%). ( E ) TRAP staining image of tibial subchondral bone at 0, 2, 4, and 8 weeks after ACLT. Scale bar, 100 (top) and 50 μm (bottom). ( F ) Micro-CT quantitative analysis of tibial subchondral bone, SBP.th (mm). ( G ) Quantitative analysis of TRAP-positive cells in subchondral bone marrow. ( H ) Representative images of LPL protein immunohistochemistry in tibial subchondral bone at 0, 2, 4, and 8 weeks after ACLT. Scale bar, 100 (top) and 50 μm (bottom). ( I ) Quantitative analysis of LPL + osteoclast in subchondral bone marrow. ( J ) Representative images of the double staining of LPL and TRAP in tibial subchondral bone at 0, 2, 4, and 8 weeks after ACLT. Scale bar, 50 μm. ( K ) Quantitative analysis of LPL + osteoclasts in subchondral bone. n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Then, hematoxylin and eosin (H&E; G1005-500ML, Servicebio, Wuhan, China), Safranin O and fast green (G1053-100ML Servicebio, Wuhan, China), and TRAP staining (G1050-50T, Servicebio, Wuhan, China) were done according to regular procedures ( , ).

Techniques: Staining, Micro-CT, Immunohistochemistry, Double Staining

Journal: Science Advances

Article Title: Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

doi: 10.1126/sciadv.abo7868

Figure Lengend Snippet: ( A ) Representative micro-CT 3D images of tibia subchondral bone of Lcp1 −/− mice and WT littermates at 2 and 4 weeks after ACLT. Scale bar, 500 μm. ( B and C ) Micro-CT quantitative analysis of tibial subchondral bone: BV/TV (%) (B) and SBP.th (mm) (C). ( D ) TRAP staining image of tibial subchondral bone of Lcp1 −/− mice and WT littermates at 2 and 4 weeks after ACLT. Scale bar, 50 μm. ( E ) Quantitative analysis of TRAP + cells in subchondral bone marrow between Lcp1 −/− mice and WT littermates. ( F ) Safranin O/Fast Green staining of Lcp1 −/− mice and WT littermates’ knee articular cartilage. Scale bar, 100 μm. ( G ) OARSI grade of knee articular cartilage. ( H ) Representative images of COL II immunohistochemistry in tibial articular cartilage of LPL −/− mice and WT littermates at 4 and 8 weeks after ACLT. Scale bar, 20 μm. ( I ) Quantitative analysis of COL II + area in articular cartilage. ( J ) Representative images of MMP13 immunohistochemistry in tibial articular cartilage of Lcp1 −/− mice and WT littermates at 4 and 8 weeks after ACLT. Scale bar, 20 μm. ( K ) Quantitative analysis of MMP13 + area in articular cartilage. n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Then, hematoxylin and eosin (H&E; G1005-500ML, Servicebio, Wuhan, China), Safranin O and fast green (G1053-100ML Servicebio, Wuhan, China), and TRAP staining (G1050-50T, Servicebio, Wuhan, China) were done according to regular procedures ( , ).

Techniques: Micro-CT, Staining, Immunohistochemistry

Journal: Science Advances

Article Title: Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression

doi: 10.1126/sciadv.abo7868

Figure Lengend Snippet: ( A ) Representative micro-CT 3D images of tibia subchondral bone of WT mice with Oxy A or normal saline at 2 and 4 weeks after ACLT. Scale bar, 500 μm. ( B ) TRAP staining images of tibial subchondral bone of WT mice with Oxy A or normal saline at 2 and 4 weeks after ACLT. Scale bar, 100 (top) and 50 μm (bottom). ( C ) Knee articular cartilage Safranin O/Fast Green staining of WT mice with Oxy A or normal saline. Scale bar, 100 μm. ( D ) Representative images of COL II immunohistochemistry in tibial articular cartilage of WT mice with Oxy A or normal saline at 4 and 8 weeks after ACLT. Scale bar, 20 μm. ( E ) Representative images of MMP13 immunohistochemistry in tibial articular cartilage of WT mice with Oxy A or normal saline at 4 and 8 weeks after ACLT. Scale bar, 20 μm. ( F to I ) Micro-CT quantitative analysis of tibial subchondral bone: BV/TV (%) (F), SBP.th (mm) (G), Tb.sp. (mm) (H), and Tb.pf (1/mm) (I). ( J ) Quantitative analysis of TRAP + cells in subchondral bone. ( K ) OARSI grade of knee articular cartilage. ( L ) Quantitative analysis of COL II + area in articular cartilage. ( M ) Quantitative analysis of MMP13 + area in articular cartilage. n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Then, hematoxylin and eosin (H&E; G1005-500ML, Servicebio, Wuhan, China), Safranin O and fast green (G1053-100ML Servicebio, Wuhan, China), and TRAP staining (G1050-50T, Servicebio, Wuhan, China) were done according to regular procedures ( , ).

Techniques: Micro-CT, Saline, Staining, Immunohistochemistry

Journal: iScience

Article Title: Basic-helix-loop-helix family member e41 suppresses osteoclastogenesis and abnormal bone resorption disease via NFATc1

doi: 10.1016/j.isci.2024.109059

Figure Lengend Snippet:

Article Snippet: Mature osteoclasts were fixed in 4% paraformaldehyde at room temperature for 20 minutes, soaked in PBS containing 0.5% Triton-X for 30 minutes, and stained with tartrate-resistant acid phosphatase (TRAP) solution (Servicebio, Cat#G1050) at 37°C for 30 minutes.

Techniques: Virus, Over Expression, Plasmid Preparation, Recombinant, Immunoprecipitation, Protease Inhibitor, Polymer, Reverse Transcription, SYBR Green Assay, RNA Extraction, Transfection, Purification, Isolation, Software, Micro-CT, Imaging, Real-time Polymerase Chain Reaction, Inverted Microscopy, Membrane, Magnetic Beads

Journal: Journal of Orthopaedic Translation

Article Title: Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model

doi: 10.1016/j.jot.2020.08.005

Figure Lengend Snippet: TRAP(tartrate-resistant acid phosphatase) staining showed that there were less osteoclasts(black arrow) on the surface of trabeculae in normal group and zoledronic acid-treated group than them in other groups. (A-D ​= ​Normal group; E-H=model group; I-L= Normal saline group; M-P ​= ​zoledronic acid-treated group); (NS= Normal saline); (bar:4× ​= ​500 ​μm, 10× ​= ​100 ​μm, 20× ​= ​100 ​μm, 40× ​= ​50 ​μm).

Article Snippet: TRAP staining suit (G1050,Servicebio), Hematoxylin dye(G1004,Servicebio), The differentiation of liquid (G1005-3,Servicebio), the blue liquid(G1005-4,Servicebio).

Techniques: Staining, Saline